Key result
RT-PCR detected Enteroviruses in 13% (30/225) and Adenoviruses in 8% (18/225) of stool specimens from acute flaccid paralysis patients that were previously negative by cell culture.
Why the study?
Some enteroviruses do not grow in conventional cell lines, which are laborious and time-consuming, creating a need for more sensitive and faster methods to detect enteroviruses in AFP cases.
Does Real-Time PCR improve the detection of Enteroviruses and Adenoviruses in cell culture-negative stool specimens of patients with acute flaccid paralysis?
Cross-Sectional (n=225)
No
Does Real-Time PCR improve the detection of Enteroviruses and Adenoviruses in cell culture-negative stool specimens of patients with acute flaccid paralysis?
RT-PCR improves the detection of Enteroviruses and Adenoviruses in AFP patients whose stool samples are negative by conventional cell culture, highlighting the need to replace cell culture with molecular methods for virological surveillance.
Answers are grounded in this paper and cite their sources.
RT-PCR may improve enterovirus/adenovirus detection in culture-negative AFP stools; leaves open whether molecular methods should replace cell culture.
In the era of polio eradication, acute flaccid paralysis (AFP), a major clinical presentation of poliovirus infection, continues to pose a public health problem.Several infectious agents comprising different families of viruses have been isolated from cases of AFP.Non-polio Enteroviruses (NPEVs) are considered to be the main infectious agents in AFP cases; further investigations have shown the isolation of Adenovirus from the stools of these patients.However, some Enteroviruses do not grow in the conventional cell lines used for the detection of Enteroviruses which is laborious and time consuming.Thus, more sensitive and faster methods are urgently needed for accurate detection of Enteroviruses in stool samples from AFP cases.
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A 2023 study conducted a cross-sectional in Acute flaccid paralysis (AFP) (n=225). Real-Time PCR (RT-PCR) vs. Cell culture was evaluated on Detection of Enteroviruses in cell culture-negative stool specimens. RT-PCR detected Enteroviruses in 13% (30/225) and Adenoviruses in 8% (18/225) of stool specimens from acute flaccid paralysis patients that were previously negative by cell culture.
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