EndoTime: non-categorical timing estimates for luteal endometrium

Julia Lipecki; A. E. Mitchell; Joanne Muter; Emma S. Lucas; Komal Makwana; Katherine Fishwick; Joshua Odendaal; Amelia Hawkes; Pavle Vrljicak; Jan J. Brosens; Sascha Ott

doi:10.1093/humrep/deac006

January 15, 2022Human ReproductionOpen Access

EndoTime: non-categorical timing estimates for luteal endometrium

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Key Points

To evaluate whether continuous, non-categorical modeling of a small panel of genes can improve the timing accuracy of luteal phase endometrial biopsies compared with urinary ovulation testing.
Measured gene expression using RT-qPCR across 664 endometrial biopsies collected 4–12 days post-positive home ovulation test, plus 36 samples evaluated by both RT-qPCR and RNA-sequencing.
Developed 'EndoTime', an iterative computational algorithm modeling temporal profiles of six target genes (IL2RB, IGFBP1, CXCL14, DPP4, GPX3, and SLC15A2) to continuously refine biopsy timing estimates.
EndoTime ordering generated sharper temporal expression profiles for held-out validation genes compared to patient-reported timing (GPX3: P < 0.005; CXCL14: P < 2.7e-6; DPP4: P < 3.7e-13).
EndoTime estimates demonstrated high consistency across RT-qPCR and RNA-sequencing platforms (Pearson R2 = 0.687, P = 8.6e-10).
Estimated timings showed no significant divergence between control participants and individuals with recurrent pregnancy loss or recurrent implantation failure (P > 0.05).

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Implication

Observational development study reveals a continuous six-gene timing model for luteal endometrial biopsies, indicating improved accuracy over urinary ovulation tests.

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Cite This Study

Lipecki et al. (2022) studied this question.

synapsesocial.com/papers/6a9a0ca24b8eac9b751d7b74 https://doi.org/10.1093/humrep/deac006

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EndoTime: non-categorical timing estimates for luteal endometrium

Key Points

No abstract yet

Implication

Discussion

Cite This Study

EndoTime: non-categorical timing estimates for luteal endometrium

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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