The membrane lipocarbohydrate antigen (lipoteichoic acid analogue) of Clostridium difficile has been purified by aqueous phenol extraction and Sepharose 6B chromatography. After analysis by polyacrylamide gel electrophoresis (PAGE) and immunoblotting it has been shown to consist of a series of components of differing Mr. It appears as a regularly spaced ladder pattern similar to those shown for the lipopolysaccharide (LPS) of many Gram-negative bacteria.
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Sharp et al. (1986) studied this question.
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