Investigating the effect of polarity of stationary and mobile phases on retention of cannabinoids in normal phase liquid chromatography

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This work reports about a screening of four adsorbents with different polarity employed for the separation of the main phytocannabinoids contained inCannabis sativaL., under normal phase liquid chromatography (NPLC). The effect of polarity and type of interaction mechanisms of the adsorbents (namely Si-, CN-, Diol-, and NH2-based SPs) on retention has been investigated under a variety of conditions either by using different combinations of apolar solvents (heptane or hexane) and alcohols (ethanol or isopropanol). The columns have also been employed for the separation of a real cannabis sample.