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An encephalitogenic protein was extracted from myelin and labelled with 131I. The binding of this radioactive protein to myelin was measured in vitro. It was found that Cu++ enhanced the binding, but that Al+++, Ca++, Mg++, Zn++, Mn++, Na+, and K+ did not. After binding, the radioactive protein was extracted from myelin and shown to be identical with the original protein.
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Moscarello et al. (1968) studied this question.
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