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Somatic carrot cells in culture divide to produce undifferentiated preglobular proembryos which exhibit a wide variety of segmentation patterns. One or more globular proembryos, which exhibit normal histological zonation, may develop from single preglobular proembryos. Regeneration of normal embryos from cultured cells grown on media containing only minerals, sucrose, vitamins, and 2,4-dichlorophenoxyacetic acid suggests that the embryo sac may have less of a formative role than currently ascribed to it.
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Halperin et al. (1965) studied this question.
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