Key result
Optical techniques for measuring AAV full-to-total ratio showed <10% SD between laboratories, whereas AUC had ≈25% SD and PCR-ELISA demonstrated poor reproducibility.
Why the study?
Therapeutic safety and efficacy of recombinant AAV depend on correct DNA encapsidation characterized by the full-to-total ratio, but interlaboratory and intertechnique variations required evaluation.
Population
Recombinant AAV5 and AAV8 serotypes
Comparison
SV-AUC, SEC-MALS, cryo-EM, dual-wavelength UV spectrophotometry, and ELISA coupled with quantitative PCR
Design
Interlaboratory and intertechnique comparative study
Authors
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Optical methods enable reliable AAV capsid quantification across labs; leaves open standardization of PCR/ELISA and AUC for clinical vector use.
Optical techniques provide the most reproducible interlaboratory measurements for AAV full-to-total capsid ratios, whereas PCR/ELISA and AUC require further standardization.
Lehman et al. (2024) studied Adeno-Associated Virus (AAV) vector characterization. Measurement methods for full-to-total (FTT) ratio was evaluated on Interlaboratory and intertechnique variations of measurement methods for FTT. Optical techniques for measuring AAV full-to-total ratio showed <10% SD between laboratories, whereas AUC had ≈25% SD and PCR-ELISA demonstrated poor reproducibility.
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