A high throughput strategy for traditional Chinese medicine active compound screening based on Raman spectroscopy

• Single-cell Raman technology was applied to screen the critical active compounds in AGNHP. • Berberine, baicalein, epiberberine, and cholic acid in AGNHP may play a more critical role in treatment of stroke. • We developed an integrated strategy combining network pharmacology, Raman spectroscopy, and machine learning for efficient screening of critical active ingredients in TCM.