A Trichoderma longibrachiatum transformant has been constructed constitutively expressing the homologous egl1 gene under the control of the Aspergillus nidulans glyceraldehyde phosphate dehydrogenase gene ( gpdA ) promoter. Consequently, the egl1 gene can be expressed in glucose-containing media. The egl1 product (EGL1) has been purified from such a medium. The physicochemical and kinetic properties of EGL1, which has both endoglucanase and xylanase activities, have been determined. Analysis of the effects of certain enological factors such as temperature, pH, and glucose, ethanol, and SO 2 concentrations on the enzyme's activities indicates that it can be used in wine production.
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Ganga et al. (1997) studied this question.
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