Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica

To evaluate the phytochemical properties, enzyme properties of an endophytic fungi from Centella asiatica plant. Endophyte was isolated by using potato dextrose agar medium from Centella asiatica plant. The qualitative chemicals screening were performed for the ethyl acetate extracts of fungi. Then the phytochemicals like phenols and flavonoids were quantitatively estimated. Then the ability of the fungal culture to produce different enzymes was analysed. Finally, High performance thin layer Chromatography was performed for alkaloid and flavonoid profiles of fungal extract. A Penicillium sp. was isolated from Centella asiatica plant and the fungus was identified by 18S rRNA analysis. The sequence of the fungus was submitted in NCBI with accession number (HM068965) and name Penicillium sp.nirjan22. The qualitative chemicals analysis of the fungus showed positive results for alkaloids, phenols, flavonoids, tannin and glycosides. It was found that the fungal crude extract contained 2.76g of phenol and 0.275 g of flavonoid. The Penicillium sp.nirjan22 produced cellulase enzyme. The HPTLC analysis of the ethyl acetate extract of fungi inferred that it contain both alkaloid and flavonoid. The present study inferred that Penicillium sp. nirjan22 contain many bioactive compounds which can further isolate and check in vitro and in vivo models against different human ailments.