Research Progress of Virus-Induced Gene Silencing and its Application in Gramineae

With the proliferation of genome sequence information, biological science research has entered the era of big data, but how to annotate the function of genome information has become an important research goal. Virus-induced gene silencing (VIGS) is a powerful tool that has been used to study key genes in multiple plant growth and development processes. This technology uses the innate antiviral defense system of plants. And generates small interfering RNA (siRNA) in the plant by inserting the target gene fragment into the viral vector, so that the transcript of the endogenous gene of the plant becomes a target for degradation, resulting in the expression of the target gene being down-regulated. This paper systematically reviews the development, mechanism, and advantages of VIGS technology, focuses on the vectors that can be applied in grasses, and finally discusses the limitations and development trend of VIGS technology in the application of Gramineae.