Regulation of output current of L-lactate sensors based on alternate deposition of avidin and biotinylated lactate oxidase on electrode surface through avidin/biotin complexation.

An alternate and repeated deposition of avidin and biotinylated lactate oxidase (LOx) gave a thin film on the surface of platinum electrode. Amperometric response of L-lactate sensors thus prepared was enhanced stepwise by increasing the number of LOx layers. The enhanced response contributed to the extension of the lower detection limit of the sensor. The response time of the sensors was satisfactorily fast (ca. 10 s), irrespective of the number of LOx layers.