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During the summer of 1990, seedlings of forsythia (Forsythia viridissima), grown in pots in a production nursery in Campania (Italy), showed symptoms of decline associated with root and crown rot. Phytophthora nicotianae of A 2 mating type was isolated from decayed tissues. Identification of the isolate was based on both morphological and physiological characters, and on the electrophoretic pattern of total native mycelial proteins. P. nicotianae was confirmed as the causal agent of this decline by fulfilling Koch's postulates. Seedlings of forsythia inoculated with the P. nicotianae isolate developed symptoms identical to those observed in natural infections
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Santa Olga Cacciola (1994) studied this question.
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