Collagen type 1 promotes survival of human breast cancer cells by overexpressing Kv10.1 potassium and Orai1 calcium channels through DDR1-dependent pathway

// Mehdi Badaoui 1 , Cloé Mimsy-Julienne 1 , Charles Saby 2 , Laurence Van Gulick 2 , Marta Peretti 1 , Pierre Jeannesson 2 , Hamid Morjani 2 and Halima Ouadid-Ahidouch 1 1 Laboratory of Cellular and Molecular Physiology, EA4667, University of Picardie Jules Verne, Amiens, France 2 Extracellular Matrix and Cellular Dynamics, Faculty of Pharmacy, MEDyC Centre National de la Recherche Scientifique UMR7369, Reims University, Reims, France Correspondence to: Halima Ouadid-Ahidouch, email: halima.ahidouch-ouadid@u-picardie.fr Keywords: Kv10.1; Orai1; tumour microenvironment; survival; breast cancer Received: October 19, 2016 Accepted: May 31, 2017 Epub: July 08, 2017 Published: May 15, 2018 ABSTRACT Collagen type 1 is among the tumor microenvironment (TM) factors, that regulates proliferation, survival, migration and invasion. Ion channels are key players in interactions between tumor cells and TM. Kv10.1 has been shown to play an essential role in breast cancer cell proliferation and migration by permitting Ca 2+ influx notably via Orai1. Here, we show that human breast cancer (BC) cells growing, in culture media completely devoid of the serum and seeded on collagen 1 coating, exhibited less apoptotic rate and a decrease in Bax expression when compared to those grown on plastic. The survival conferred by collagen 1 was completely abolished by removing extracellular Ca 2+ from the culture medium. In addition, Ca 2+ entry was increased in collagen 1 condition along with increased Kv10.1 and Orai1 expressions. Moreover, collagen 1 was able to increase co-localization of Kv10.1 and Orai1 on the plasma membrane. Interestingly, silencing of Kv10.1 and Orai1 reduced survival and Ca 2+ influx without any additive effect. This calcium-dependent survival is accompanied by the activation of ERK1/2, and its pharmacological inhibition completely abolished the increase in Kv10.1 and Orai1 expressions, activities, and the cell survival induced by collagen 1. Moreover, both Kv10.1 and Orai1 knockdown reduced ERK1/2 activation but not Akt. Finally, DDR1 silencing but not β1-integrin reduced the collagen induced survival, ERK1/2 phosphorylation and the expression of Kv10.1 and Orai1. Together these data show that the Kv10.1/Orai1 complex is involved in BC cell survival and this is dependent on collagen 1/DDR1 pathway. Therefore, they represent a checkpoint of tumor progression induced by the tumor microenvironment.