Exposure and Risk Assessment for Operator Exposure to Insecticide Acetamiprid during Water Melon Cultivation in Greenhouse using Whole Body Dosimetry

수박 시설 재배에서 acetamiprid 수화제의 살포액 조제 및 살포 시 농작업자의 피부 및 호흡 노출량을 측정하였다. 국내 최초로 피부 노출은 전신노출법을 이용하였으며, 폴리에스테르와 면이 함유된 겉작업복과 면으로 된 내복을 사용하여 측정하였다. 손 노출은 nitrile 장갑을 끼고 조제/살포 후 장갑 및 손을 세척하여 측정하였고, 머리의 노출은 세척액으로 적신 거즈로 얼굴/목을 닦아서 측정하였다. 호흡 노출은 개인용 공기펌프와 IOM sampler (glass fiber filter)를 작업자 어깨에 고정하여 2 L/min으로 공기를 포집하여 측정하였다. 분석법의 정량한계는 2.5 ng/mL이었고, 각 노출 시료의 matrix matched 표준용액의 직선성은 0.99 이상이었다. 재현성은 C.V 8.7% 이하였으며, 각 노출시료의 회수율은 70~119%, 포장회수율은 77~95%이었다. 조제 시 손 노출량( 229.7μg )이 살포시 노출량( 20.9μg )의 10배 이상의 결과를 보였다. 살포시 전체 피부 노출량은 1207.4μg 이었고, 이 중 정강이의 노출량은 1132.1μg 로 전체의 93.8 %를 차지하였다. 호흡 노출량은 조제 및 살포 시 모두 검출되지 않았다. 위해성평가를 위한 MOS는 한국 남자 평균 체중(70 kg)과 농작업자 노출 허용량( 124μg/kg/day )을 고려하여 산정하였다. MOS 값 산출결과, 100이상으로 위해성이 낮음을 확인하였다. Assessment for operator's dermal and inhalation exposure to acetamiprid during cultivation of water melon in greenhouse was carried out. For dermal exposure measurement, whole body dosimetry (WBD) was performed as the first trial in Korea. WBD consists of cotton/polyester outer clothes and cotton inner clothes. Hand exposure was measured by washing of nitrile gloves and hands while head exposure was monitored by face/neck wipe technique. Inhalation exposure was monitored with personal air sampling pumps and IOM sampler (glass fiber filter). Analytical limit of quantitation was 2.5 ng/mL. Good reproducibility (C.V < 8.7%), linearity ( R² > 0.99) and recovery (70~119%) were obtained. Field recovery of acetamiprid was 77~95%. During mixing/loading, hand exposure of acetamiprid was about 10 times ( 229.7μg ) more than that of application case ( 20.9μg ). During application, total dermal exposure was 1207.4μg . Exposure of lower legs was 1132.1μg , which is 93.8% of the total dermal exposure. Inhalation exposure during mixing/loading and application was not detected. Margin of safety (MOS) was calculated for risk assessment using male Korean average body weight (70 kg) and acceptable operator exposure level ( 124μg/kg/day ) to give 140, suggesting that health risk of operator during treatment of acetamiprid for water melon in greenhouse could be safe.