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An advanced biosensor based on a closed bipolar electrode (BPE)–electrochemiluminescence (ECL) strategy is proposed for cancer cell adhesion, specific recognition, and detection with dual biomarkers. The system consists of a two‐channel polydimethylsiloxane (PDMS) chip (sensing channel and reporting channel) connected through a U‐shaped indium tin oxide BPE at a glass surface. A sandwich‐type cancer cell detection model is developed at the cathode of the BPE with two recognition molecules, folic acid (FA) and an aptamer. On the basis of the high affinity between FA and the folate receptor that is overexpressed at a cancer cell membrane as well as the excellent conductivity of graphene–Au conjugates, this strategy could achieve the sensitive detection of HL‐60 cells, with a limit as low as 18 cells in 30 μL of cell suspension. Control experiments confirm that the sandwich assay specifically captures cancer cells and recognizes target cells.
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Wu et al. (2015) studied this question.
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