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September 4, 2026ChemistrySelect

Green Synthesis of Silver Nanoparticles Using Ammoides pusilla Extract: Optimization, Characterization, and Antimicrobial Activity

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Authors

KKKhadidja KhaldounNANadia AissaouiBRBoudoumi Redouane

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Overview

In vitro study demonstrates potent antimicrobial activity of biosynthesized silver nanoparticles against bacterial and fungal pathogens, highlighting a sustainable plant-based antimicrobial approach.

Key Points

  • To optimize the eco-friendly synthesis of silver nanoparticles using Ammoides pusilla extract and characterize their physical properties and antimicrobial effectiveness.
  • Investigated the influence of temperature, pH, reaction time, silver nitrate concentration, and plant extract concentration on silver nanoparticle synthesis.
  • Characterized the biosynthesized nanoparticles using UV–visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), FTIR, and thermogravimetric analysis (TGA).
  • Assessed antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Bacillus cereus, and Staphylococcus aureus using zone-of-inhibition assays.
  • Optimal synthesis conditions were achieved at 80°C, pH 9, and 2 hours of reaction time using 10% (w/v) Ammoides pusilla extract and 3 mM silver nitrate.
  • Nanoparticles were predominantly spherical with TEM-measured diameters of 12–18 nm and an XRD crystallite size of 17 nm.
  • Biosynthesized silver nanoparticles generated inhibition zones of 20.33 ± 0.58 mm for E. coli, 20.67 ± 0.58 mm for P. aeruginosa, 30.33 ± 0.58 mm for C. albicans, 25.67 ± 0.58 mm for B. cereus, and 21.00 ± 1.00 mm for S. aureus.

Cite This Study

Khaldoun et al. (2026) studied this question.

synapsesocial.com/papers/6a9a85fa5d9e33f25c6316cdhttps://doi.org/10.1002/slct.74285
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