Molecular Detection of blaTEM and blaSHF in Diarrhoegenic Escherichia coli Isolated from Egyptian Children

Introduction: Escherichia coli (E.coli) is a common bacterial pathogen for acute diarrhea in children. The aim of the present study was to determine the prevalence mainly of TEM and SHV-beta lactamase-encoding genes responsible for extended spectrum beta lactamase (ESBL) production amongst diarrheagenic E. coli species as these strains are reported to have been increased in recent years. Methodology: The study included children with acute diarrhea presented in Mansoura University children hospital during the period from January 2011 till Jun 2014. In the microbiological laboratory stool samples were cultured and the isolated colonies were identified by standard biochemical reactions and by multiplex polymerase chain reaction (PCR) for identification of diarrheagenic E.coli strains. In vitro susceptibility testing of all isolates was performed using the discs diffusion method. E.coli isolates were subjected to testing to detect the possible presence of SHV, and TEM genes by conventional PCR.