Key result
Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU of Mahoney type 1 poliovirus in a 0.2 ml inoculum, suggesting utility for environmental enterovirus detection.
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Buffalo green monkey kidney cell shaker cultures can effectively detect small quantities of enteroviruses from environmental sources.
Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.
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Goldstein et al. (1982) studied Enterovirus detection. Buffalo green monkey kidney cell shaker cultures vs. Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures was evaluated on Detection of enteroviruses (poliovirus, coxsackievirus, echovirus). Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU of Mahoney type 1 poliovirus in a 0.2 ml inoculum, suggesting utility for environmental enterovirus detection.
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