Key result
Extraction with 10% sarkosyl and differential centrifugation efficiently yields 5-10 micrograms of scrapie-associated fibril protein per hamster brain, copurifying with scrapie infectivity.
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A rapid and efficient method using 10% sarkosyl and differential centrifugation can enrich SAF-protein from scrapie hamster brains.
Scrapie hamster brains contain at least 5-10 micrograms of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differential centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.
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Hilmert et al. (1984) studied this question. Extraction with 10% sarkosyl and differential centrifugation efficiently yields 5-10 micrograms of scrapie-associated fibril protein per hamster brain, copurifying with scrapie infectivity.
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