Fast Consecutive Intramolecular Processes Involving Protein and Fe(III) in Ferri‐Cytochrome‐c in Aqueous Solution

The reaction of H atoms (produced by nanosecond pulse radiolysis) with ferri‐cytochrome‐c in aqueous solution results in the second order formation of an absorption spectrum due to the addition of H atoms to the enzyme protein. This spectrum is not specific to the iron moiety. The rate constant of the addition is 1×1010M−1 sec−1. This is followed by two first order intramolecular processes with specific rates of ∼ 1×105 sec−1 and ∼ 2×104 sec−1. In these processes, specific spectra related to the α and β bands of ferro‐cytochrome‐c appear. The results are interpreted to show reduction equivalent transfer through the protein to the iron moiety.