Key result
PLA/PEI microspheres loaded with DNA vaccine achieved an 87.5% protection rate against foot-and-mouth disease in guinea pigs, significantly higher than the pure DNA vaccine.
Why the study?
Nanotechnology provides an impetus to formulate microsphere or nanosphere delivery systems for treating infectious diseases in animals.
PLA/PEI microspheres demonstrate high transfection efficiency and significantly improve the protection rate of DNA vaccines against foot-and-mouth disease in a guinea pig model.
May enhance DNA vaccine efficacy in guinea pig models; leaves open translation to other species or clinical use.
Nanotechnology represents a new impetus for biomedical research applications, especially using nanotechnology to formulate microspheres or nanospheres based delivery system for treatment of infectious diseases in animals. In this work, polylactic acid (PLA) microspheres with an average size of 156 nm were prepared by combining emulsion polymerization coupled with emulsion-solvent evaporation. Coating with polyethylenimine (PEI) polymers increased the surface charges of the resulting PLA/PEI microspheres, thus enabled plasmid DNA to adsorb tightly to the microspheres. As expected, the plasmid DNA was successfully transferred into the pig kidney-15 cells with high transfection efficiency. In addition, the protection rate of PLA/PEI microspheres loaded with DNA vaccine against foot-and-mouth disease in guinea pigs reached 87.5%, which was significantly higher than that of the pure DNA vaccine group. These results indicated that PLA/PEI microspheres were expected to be an effective delivery system for DNA vaccines.
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Ren et al. (2021) studied Foot-and-mouth disease. PLA/PEI microspheres loaded with DNA vaccine vs. Pure DNA vaccine was evaluated on Protection rate against foot-and-mouth disease. PLA/PEI microspheres loaded with DNA vaccine achieved an 87.5% protection rate against foot-and-mouth disease in guinea pigs, significantly higher than the pure DNA vaccine.
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