We have fabricated aminopropyltriethoxysilane (APTES)-functionalized nanoporous polymeric gratings by combining holographic interference patterning and APTES-functionalization of the pre-polymer syrup. The APTES facilitates the immobilization of biomolecules onto the polymeric grating surfaces. The successful detection of multiple biomolecules (biotin, steptavidin, biotinylated anti-rabbit IgG, and rabbit-IgG) indicates that the functionalized nanoporous polymeric gratings can act as biosensing platforms which are label-free, inexpensive, and applicable as high-throughput assays.
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Hsiao et al. (2007) studied this question.
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