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Circularly polarized emission (CPE) is observed from achiral fluorescent molecules dissolved in optically inactive solvents. An extensive investigation is carried out to determine whether or not the signal observed is a true solvent-induced CPE signal. As a result, the following three decisive factors responsible for the origin of the artifact in CPE spectroscopy are presented: (1) the amount of linear polarization in emission; (2) the residual static birefringence of the optical system due mainly to an electro-optical modulator; and (3) the second harmonics response of the lock-in amplifier used in the electronic system.
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Shindo et al. (1985) studied this question.
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