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September 27, 2022Cytometry Part AOpen Access

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets of bottlenose dolphins ( Tursiops truncatus )

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Authors

MFMar Felipo‐BenaventAMAlicia Martínez‐RomeroCRConsuelo Rubio‐Guerri

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Overview

Laboratory study demonstrates whole-blood kinetic assay of platelet calcium mobilization in bottlenose dolphins, highlighting a sensitive tool for early veterinary diagnosis.

Key Points

  • To adapt a human whole-blood flow cytometric assay for measuring intracellular calcium mobilization during platelet activation in bottlenose dolphins (Tursiops truncatus).
  • Adapted an established human kinetic assay to dolphin whole blood using a no-wash, no-lyse protocol under near-physiological conditions.
  • Employed the calcium-sensitive fluorescent dye Fluo-4AM combined with an anti-CD41-PE antibody clone that cross-reacts with dolphin platelets.
  • Measured real-time kinetic changes and intensity of intracellular calcium mobilization following exposure to platelet agonists via flow cytometry.
  • Successfully established a no-wash, no-lyse kinetic assay that monitors real-time intracellular calcium mobilization in dolphin platelets.
  • Demonstrated reliable identification of dolphin platelets in whole blood using the CD41-PE antibody clone and Fluo-4AM indicator.
  • Detected agonist-induced signal transduction responses, providing a functional method to evaluate early alterations in marine mammal hemostasis.

Cite This Study

Felipo‐Benavent et al. (2022) studied this question.

synapsesocial.com/papers/6a9b139dd17f9b912e91c959https://doi.org/10.1002/cyto.a.24693
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