What question did this study set out to answer?

To evaluate methods for measuring Staphylococcus aureus vaccine candidate antigen expression and the corresponding host immune response during clinical infection.

February 26, 2015PLoS ONEOpen Access

Evaluation of Approaches to Monitor Staphylococcus aureus Virulence Factor Expression during Human Disease

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Authors

WRWouter RozemeijerNoordwest Ziekenhuisgroep PFPamela S. FinkPfizer (United States)EREduardo A. Peña RojasMcMaster Children's Hospital

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Implication

Observational study reveals active expression of vaccine antigens in patients with Staphylococcus aureus infections, supporting their use in prophylactic vaccine designs.

Key Points

To evaluate methods for measuring Staphylococcus aureus vaccine candidate antigen expression and the corresponding host immune response during clinical infection.
Enrolled 51 hospitalized patients with diagnosed S. aureus infections, comprising 27 wound and 24 bloodstream infections, to analyze invasive and nasal carriage isolates.
Assessed bacterial antigen expression directly via quantitative reverse-transcriptase PCR (qRT-PCR) and indirectly by measuring acute and convalescent antibody titers using a competitive Luminex immunoassay.
All genotypically diverse clinical isolates harbored genes encoding the SA4Ag vaccine components (ClfA, MntC, and capsular polysaccharides), with nasal carriage identified in 55% of patients (64% matched invasive strains).
Bacterial RNA transcripts for ClfA, MntC, and capsular polysaccharides were confirmed by qRT-PCR in swab samples expressing the triosephosphate isomerase housekeeping gene.
Serum antibody titers against target antigens rose significantly from the acute to convalescent phase of infection.

Cite This Study

Rozemeijer et al. (2015) studied this question.

synapsesocial.com/papers/6a9b172ec0630401c35094bb https://doi.org/10.1371/journal.pone.0116945

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Implication

Observational study reveals active expression of vaccine antigens in patients with Staphylococcus aureus infections, supporting their use in prophylactic vaccine designs.

Evaluation of Approaches to Monitor Staphylococcus aureus Virulence Factor Expression during Human Disease

Discussion

Member takes

Implication

Key Points

Cite This Study

Evaluation of Approaches to Monitor Staphylococcus aureus Virulence Factor Expression during Human Disease

Discussion

Member takes

Implication

Key Points

Cite This Study

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