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SUMMARY A method for preparing 125I-labeled AS anti-August rat transplantation alloantibody is described. After absorption and elution from membranes prepared from a spontaneous August myelogenous leukaemia, antibody was partially purified on Sephadex G-200 and iodinated with 125I. After iodination, further purification was achieved by DEAE-chromatography. The final labeled preparation had a specificity ratio of 25–40:1 when the amount bound to August target lymphocytes was compared to that bound to AS target lymphocytes. The specific binding activity could be absorbed by August cells and blocked by unlabeled antibody. The technical problems in obtaining a satisfactory 125I-labeled preparation of AS anti-August antibody are discussed in detail, with a view to providing information applicable to labeling transplantation antibodies in other systems.
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Shumak et al. (1973) studied this question.
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