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Summary: A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV‐transparent solvent system. Calibrating by the extinction coefficients (A210) of D‐glucosamine and N‐acetyl‐D‐glucosamine, DD values (24–88%) were computed numerically. The results correlated well (R2 = 0.9805, n = 50) with those obtained by solid‐state 13C NMR. Comparison of the results obtained by the proposed UV method and solid‐state 13C NMR. magnified image Comparison of the results obtained by the proposed UV method and solid‐state 13C NMR.
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Hsiao et al. (2004) studied this question.
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