Key result
Differentiation of human myoblasts to multinuclear myotubes displayed a linear increase in PTPIP51 expression, which was partly reversible by resubmission to proliferation conditions.
PTPIP51 expression is strongly associated with differentiation in human skeletal muscle cells.
PTPIP51 may mark myoblast differentiation in culture; leaves open its role in human muscle regeneration or disease.
Protein tyrosine phosphatase-interacting protein 51 (PTPIP51) expression was analyzed in proliferating and differentiating human myogenic cells cultured in vitro. Satellite cell cultures derived from four different individuals were used in this study. To analyze the expression of PTPIP51, myoblasts were cultured under conditions promoting either proliferation or differentiation. In addition, further differentiation of already-differentiated myobtubes was inhibited by resubmitting the cells to conditions promoting proliferation. PTPIP51 protein and mRNA were investigated in samples taken at defined time intervals by immunostaining, immunoblotting, in situ hybridization, and PCR. Image analyses of fluorescence immunostainings were used to quantify PTPIP51 in cultured myoblasts and myotubes. Myoblasts grown in the presence of epidermal and fibroblast growth factors (EGF and FGF), both promoting proliferation, expressed PTPIP51 on a basic level. Differentiation to multinuclear myotubes displayed a linear increase in PTPIP51 expression. The rise in PTPIP51 protein was paralleled by an augmented expression of muscle-specific proteins, namely, sarcoplasmic reticulum Ca(2+) ATPase and myosin heavy-chain protein, both linked to a progressive state of myotubal differentiation. This differentiation-induced increase in PTPIP51 was partly reversible by resubmission of differentiated myotubes to conditions boosting proliferation. The results clearly point toward a strong association between PTPIP51 expression and differentiation in human muscle cells.
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Barop et al. (2009) studied In vitro human skeletal muscle cell culture (n=4). Differentiation medium (absence of EGF and FGF) vs. Proliferation medium (EGF and FGF) was evaluated on PTPIP51 expression. Differentiation of human myoblasts to multinuclear myotubes displayed a linear increase in PTPIP51 expression, which was partly reversible by resubmission to proliferation conditions.
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