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A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.
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Belenkaya et al. (2021) studied this question.
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