What question did this study set out to answer?

To determine whether a soluble form of the envelope glycoprotein GN (GN-S) inhibits transmission of Tomato spotted wilt virus (TSWV) by Frankliniella occidentalis and to measure the persistence of this effect.

December 14, 2007Phytopathology

A Soluble Form of the Tomato spotted wilt virus (TSWV) Glycoprotein G N (G N -S) Inhibits Transmission of TSWV by Frankliniella occidentalis

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Authors

AWAnna E. WhitfieldNorth Carolina State University NKN. K. Krishna KumarTribhuvan University DRDorith RotenbergNorth Carolina State University

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Implication

Experimental study reveals that soluble viral glycoprotein GN-S reduces Tomato spotted wilt virus transmission in thrips, indicating a viable strategy to disrupt plant viral vector competence.

Key Points

To determine whether a soluble form of the envelope glycoprotein GN (GN-S) inhibits transmission of Tomato spotted wilt virus (TSWV) by Frankliniella occidentalis and to measure the persistence of this effect.
Fed thrips an acquisition access period (AAP) containing purified TSWV mixed with GN-S protein and assayed individual adult insects for transmission competence.
Supplied thrips with an AAP on GN-S protein before feeding on TSWV-infected plants, assaying individual transmission over three intervals: 2–3, 4–5, and 6–7 days post-adult eclosion.
Measured virus titers in individual insects across exposure groups using real-time reverse transcription polymerase chain reaction (RT-PCR).
Exposure to GN-S mixed with purified virus reduced the percentage of transmitting adult thrips eightfold relative to control insects.
Pre-exposure to GN-S produced a significant reduction in transmission that persisted equally across all three time intervals (2–3, 4–5, and 6–7 days post-eclosion).
Real-time RT-PCR showed a threefold reduction in the proportion of thrips infected with TSWV following GN-S pre-exposure, with non-transmitting insects exhibiting no detectable infection.

Cite This Study

Whitfield et al. (2007) studied this question.

synapsesocial.com/papers/6a9b2be60f8eed8d6069f9e1 https://doi.org/10.1094/phyto-98-1-0045

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Implication

Experimental study reveals that soluble viral glycoprotein GN-S reduces Tomato spotted wilt virus transmission in thrips, indicating a viable strategy to disrupt plant viral vector competence.

A Soluble Form of the Tomato spotted wilt virus (TSWV) Glycoprotein G N (G N -S) Inhibits Transmission of TSWV by Frankliniella occidentalis

Discussion

Member takes

Implication

Key Points

Cite This Study

A Soluble Form of the Tomato spotted wilt virus (TSWV) Glycoprotein G N (G N -S) Inhibits Transmission of TSWV by Frankliniella occidentalis

Discussion

Member takes

Implication

Key Points

Cite This Study

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