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Alloxan and H2O2 irreversibly depolarize insulinoma cells by opening a non-selective cation channel, an effect prevented by free radical scavengers.
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Alloxan and H2O2 depolarize insulin-secreting cells via a specific non-selective cation channel, providing insight into free radical-induced cellular mechanisms.
Alloxan and its auto-oxidation product hydrogen peroxide (H2O2) irreversibly depolarize insulinoma cells by opening a non-selective cation channel. The channel opened is characterized by a linear current-voltage relation with a conductance of approximately 70 pS and very slow kinetics (of the order of seconds). 2. Cells are protected against the alloxan-induced channel opening and consequent cell depolarization by the presence of H2O2 and hydroxyl radical scavengers. 3. The free radical-activated non-selective cation channel is not operative in isolated patches but can be activated by the application of beta-NAD+ to the cytoplasmic aspect of the membrane.
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Herson et al. (1997) studied this question. Alloxan and H2O2 was evaluated on Channel activation and cell depolarization. Alloxan and H2O2 irreversibly depolarize insulinoma cells by opening a non-selective cation channel, an effect prevented by free radical scavengers.
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