Elucidating the Biosynthetic Pathway for Vibralactone: A Pancreatic Lipase Inhibitor with a Fused Bicyclic β‐Lactone

Not so simple: Evidence from 13C-labeling studies, metabolite profiling, and cell-free conversion established that the bicycle skeleton of vibralactone is derived from an aryl ring moiety and both shikimate and phenylalanine pathways may contribute. VibPT was found to be potentially involved in vibralactone biosynthesis, as well as an aromatic PTase, which was first characterized in basidiomycete fungi. OPP=pyrophosphate.