Static fluorescence titration and fluorescence stopped-flow kinetic experiments o N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide-labeled troponin C showed that the molecular kinetic mechanism of the local conformational change induced by the rapid Ca2+ binding and removal reactions with the high affinity Ca2+-binding sites (sites III and IV) can be explained by the following scheme: [Formula: see text]. Numerical constants in this scheme were determined in this work.
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Iio et al. (1980) studied this question.
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