The expression profile analysis of atrial mRNA in rats with atrial fibrillation: the role of IGF1 in atrial fibrosis

Figures

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  Fig. 1. The changes of electrophysiology in AF model. (a) The electrocardiogramof sinus rhythm and AF rhythm; (b) The changes of AERP in control and AF group; (c)The AF inducibility of control and AF group. (*p < 0.05, **p < 0.01)

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  Fig. 2. The general situation of differently expressed transcripts. (a) The volcano plot of differently expressed transcripts, the red dots indicate differently expressed transcripts; (b) The heat map of differently expressed transcripts, the red indicates high expression, and the green indicates low expression; (c) The verification of random selected transcripts by qRT-PCR; (d) The GO analysis of differently expressed transcripts; (e) The KEGG analysis of differently expressed transcripts. FC:Fold Change; CPM:counts per 1 million. (*p < 0.05, **p < 0.01)

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  Fig. 3. The expression of mIGF1. (a) The expression of mIGF1 in AF group detected by qRT-PCR; (b)The expression of mIGF1 in AF group detected by Western Blotting; (c) The expression of mIGF1 after AAV infected detected by qRT-PCR; (d) The expression of mIGF1 after AAV infected detected by Western Blotting. (e) The expression level of COL1A1 increased with the prolongation of pacing time; (f) The expression level mIGF1 increased with the prolongation of pacing time. A-TP: atrial tachypacing. (*p < 0.05, **p < 0.01)

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  Fig. 4. The changes of electrophysiology after AAV. (a) The AERP changes in negative group, pacing group, pacing + mIGF1sliencing AAV group; (b) The inducibility in negative group, pacing group, pacing + mIGF1sliencing AAV group; (c) The fibrosis degree of negative group, pacing group, pacing + mIGF1sliencing AAV group by Masson staining (200×). A-TP: atrial tachypacing. (*p < 0.05, **p < 0.01)

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  Fig. 5. (a) The overview of bands after mIGF1 gene interference; (b) The relative expression of mIGF1 after mIGF1 gene interference; (c) The relative expression of CTGF and AT1R after mIGF1 gene interference; (d) The phosphorylation level of PI3K and Akt after mIGF1 gene interference; (e) The relative expression of Bcl2 and Bax after mIGF1 gene interference

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  Fig. 6. (a) The cell viability of fibroblasts after mIGF1 interfered detected by CCK8 Kit; (b) The expression of COL1A1, COL3A1 after mIGF1 interfered detected by qRT-PCR. (c) A schematic diagram to summarize the downstream singling pathway of IGF1 associated with atrial fibrosis during AF. (+) positive regulation. (*p < 0.05, **p < 0.01)