Long Noncoding RNA (lncRNA) n379519 Promotes Cardiac Fibrosis in Post-Infarct Myocardium by Targeting miR-30

Figures

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  Figure 1. LncRNA n379519 is up-regulated in infarcted myocardium and TGF-β1-induced CFs. (A) Establishment of a myocardial infarction model in SD rats. (B) RT-PCR was used to detect the expression of n379519 on day 14 and day 28. (C) Relative expression levels of n379519 in the peri-infarct region of myocardial and TGF-β1 treatment CFs. Data are expressed as mean ±SD. * P<0.05, ** P<0.01 vs. sham operation group or control group. # P<0.05, ## P<0.01 vs. MI group or TGF-β1 group.

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  Figure 2. Suppression of n379519 improved heart function and attenuates fibrotic changes of myocardium. (A) Echocardiography images of each group. (B) Ejection fraction. (C) Left ventricular fractional shortening. (D) HE staining of crosses sections of the heart. (E) Comparison of infarct size. (F) Comparison of left ventricular wall thickness. (G) Masson staining and Collagen Volume Fraction were used to compare the severity of myocardial fibrosis. (H) The mRNA expression level of collagen I. (I) The mRNA expression level of collagen III. Data are expressed as mean ±SD. * P<0.05, ** P<0.01 vs. sham operation group. # P<0.05, ## P<0.01 vs. MI group.

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  Figure 3. lncRNA n379519 functions as an miR-30 sponge in myocardial fibrosis. (A) The relative expression level of miR-30 in the peri-infract region of myocardium. (B) The relative expression level of miR-30 in TGF-β1 treatment CFs. (C) CFs was transfected with miR-30 inhibitor and the expression of n379519 was measured using RT-PCR. (D) Putative complementary sites within miR-30 and 3′-UTR of n379519 predicted using Starbase. (E) The miR-30 mimics and WT/Mut n379519 vectors were co-transfected into CFs and the probability of binding of miR-30 with the 3′-UTR of n379519 was assessed by luciferase reporter assay. Data are expressed as mean ±SD. * P <0.05, ** P<0.01 vs. sham operation group or control group or miR-NC/inhibitor group. # P<0.05, ## P<0.01 vs. MI group or TGF-β1 group.

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  Figure 4. lncRNA n379519 regulated myocardial collagen deposition by targeting miR-30. (A, B) CFs were co-transfected with si-n379519 and miR-30 inhibitor. Expressions of collagen I and collagen III were detected using RT-PCR. (C, D) Protein expressions of collagen I and collagen III in CFs transfected with si-n379519 and miR-30 inhibitor were measured by Western blot. Data are expressed as mean ±SD. * P<0.05, ** P<0.01 vs. control group. # P<0.05, ## P<0.01 vs. TGF-β1 group. & P<0.05 vs. si-n379519 group.