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September 5, 2026ACS Sustainable Chemistry & Engineering

Biorefinery of Moldy Corn for Poly(3-hydroxybutyrate) Synthesis Utilizing Halomonas sp. DYZ-4: Unsterile Craft Model and Metabolic Mechanisms

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Authors

LZLeizhen ZhaoLCLin ChenGSGaojie Sun

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Overview

Experimental study demonstrates efficient unsterile poly(3-hydroxybutyrate) synthesis from moldy corn using Halomonas sp. DYZ-4, indicating a cost-effective pathway for bioplastic production.

Key Points

  • To evaluate the feasibility and metabolic mechanisms of utilizing the halophile Halomonas sp. DYZ-4 for poly(3-hydroxybutyrate) synthesis from moldy corn in an unsterile fermentation system.
  • Characterized the fermentation performance and metabolic pathways of the halophile Halomonas sp. DYZ-4 under conventional and open (unsterile) culture conditions.
  • Pretreated moldy corn using α-amylase to generate reducing sugar feedstocks and optimized nutrient supply along with physicochemical parameters for biopolymer synthesis.
  • Enzymatic pretreatment of moldy corn with α-amylase yielded 10.72 g L–1 of reducing sugar.
  • PHB accumulation reached 6.81 g L–1 in the conventional system and 6.83 g L–1 in the unsterile open process, achieving an intracellular PHB proportion of 61%.

Cite This Study

Zhao et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd4126b95aff0620eb5e9https://doi.org/10.1021/acssuschemeng.6c04305
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