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September 5, 2026VirusesOpen Access

Heterologous Expression of Cryphonectria Hypovirus 1 in Fusarium verticillioides Reveals Stable Replication and Reduced Fumonisin Production

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Authors

SUSofía B. UllaAJAndrés G. JacquatMCMaría C. Cañizares

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Overview

Experimental study shows that heterologous Cryphonectria hypovirus 1 reduces fumonisin B1 synthesis in Fusarium verticillioides, indicating a viable virocontrol strategy for maize crops.

Key Points

  • Investigate whether the heterologous mycovirus Cryphonectria hypovirus 1 can stably infect Fusarium verticillioides and suppress fumonisin B1 production.
  • Transfected F. verticillioides protoplasts with an infectious cDNA clone of Cryphonectria hypovirus 1 (CHV1).
  • Confirmed viral integration and autonomous cytoplasmic replication using RT-PCR and cellulose-based dsRNA chromatography.
  • Evaluated fungal vegetative growth and quantified fumonisin B1 production across four independent transformants in vitro, as well as on maize in greenhouse and field settings.
  • Demonstrated stable cDNA integration into the host genome and autonomous CHV1 replication in the cytoplasm of F. verticillioides.
  • Observed significant reductions in fumonisin B1 production across all four CHV1 transformants compared to the wild-type parental strain, alongside only moderate vegetative growth changes.
  • Confirmed the stability of CHV1-mediated fumonisin B1 downregulation in maize plants under both greenhouse and field conditions.

Cite This Study

Ulla et al. (2026) studied this question.

synapsesocial.com/papers/6a9bd4346b95aff0620eba46https://doi.org/10.3390/v18090971
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