Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 20, 2013International Journal of Molecular MedicineOpen Access

Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS

View Full Paper
Ask AI
Bookmark
Share

Key result

Overexpression of Angptl4 in rat pulmonary microvascular endothelial cells exposed to LPS significantly decreased TNF-α levels (P<0.01) and inhibited LPS-induced increases in cell permeability.

Why the study?

Does Angptl4 overexpression protect rat pulmonary microvascular endothelial cells exposed to LPS?

Population

Rat pulmonary microvascular endothelial cells (RPMVECs) exposed to lipopolysaccharide (LPS)

Comparison

Transfection with Angptl4 expression vector to… vs Empty vector (pcDNA3.1-eGFP) and blank control

Design

Preclinical

Authors

YWYuxi WangMcGovern Institute for Brain ResearchHCHailong ChenEast China University of Science and TechnologyHLHailong LiQingdao Binhai University

Discussion

Loading...

Member takes

Implication

May attenuate LPS-induced endothelial injury in vitro; hypothesis-generating for acute lung injury therapy.

Structured PICO

Does Angptl4 overexpression protect rat pulmonary microvascular endothelial cells exposed to LPS?

P
Population
In vitro study using rat pulmonary microvascular endothelial cells exposed to lipopolysaccharide to model acute lung injury.
I
Intervention
Transfection with Angptl4 expression vector (pcDNA3.1-eGFP-Angptl4) to overexpress Angptl4
C
Comparator
Empty vector (pcDNA3.1-eGFP) and blank control
O
Outcome
Cell permeability, apoptosis markers (caspase-8, -9, Bax), inflammatory cytokines (TNF-α), and protein expression (p-AKT/AKT, p-Mek1/2)surrogate

Main Result

p-value: p=<0.01

Overexpression of Angptl4 exerts protective, anti-inflammatory, and anti-angiogenic effects in LPS-exposed rat pulmonary microvascular endothelial cells, suggesting a potential therapeutic target for acute lung injury.

Limitations

  • The exact mechanisms by which Angptl4 secretion influences TNF-α concentration require further investigation.
  • In vitro study design limits direct clinical translation to human acute lung injury.

Cite This Study

Wang et al. (2013) studied Acute lung injury (LPS-induced). Angptl4 overexpression (pcDNA3.1-eGFP-Angptl4 transfection) vs. Empty vector (pcDNA3.1-eGFP) and blank control was evaluated on Inflammatory cytokine (TNF-α) levels, apoptosis markers, and cell permeability (p=<0.01). Overexpression of Angptl4 in rat pulmonary microvascular endothelial cells exposed to LPS significantly decreased TNF-α levels (P<0.01) and inhibited LPS-induced increases in cell permeability.

synapsesocial.com/papers/6a9be83880a92e2a48954f36https://doi.org/10.3892/ijmm.2013.1420
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Angiopoietin-4 Promotes Glioblastoma Progression by Enhancing Tumor Cell Viability and Angiogenesis2010 · 64 citations
  2. 2Extracellular Matrix–Bound Angiopoietin-Like 4 Inhibits Endothelial Cell Adhesion, Migration, and Sprouting and Alters Actin Cytoskeleton2006 · 202 citations
  3. 3Regulation of lipid metabolism via angiopoietin-like proteins2005 · 97 citations
  4. 4Regulation of lipid metabolism via angiopoietin-like proteins2005 · 112 citations
  5. 5Cyclic AMP Blocks Bacterial Lipopolysaccharide-Induced Myosin Light Chain Phosphorylation in Endothelial Cells Through Inhibition of Rho/Rho Kinase Signaling2000 · 145 citations