Key result
Expression of a dominant negative fragment of Trp3 (NTRP3) abolished the oxidant-induced cation current and reduced membrane depolarization in porcine aortic endothelial cells.
Population
Porcine aortic endothelial cells (ECAP)
Comparison
Transient transfection to express NTRP3 followed… vs Expression of CTRP3 or untransfected cells…
Design
Preclinical
Authors
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Trp3 mediates endothelial redox sensitivity in porcine cells; hypothesis-generating for human vascular oxidant signaling and targeted therapies.
Trp proteins appear to be the molecular basis for oxidant-activated cation channels in vascular endothelium, mediating its redox sensitivity.
M. Balzer (1999) studied Oxidative stress in endothelial cells. Expression of NTRP3 (dominant negative fragment of Trp3) vs. Expression of CTRP3 (C-terminal fragment of hTrp3) was evaluated on Oxidant-induced cation current and membrane depolarization. Expression of a dominant negative fragment of Trp3 (NTRP3) abolished the oxidant-induced cation current and reduced membrane depolarization in porcine aortic endothelial cells.