Purification of Piscirickettsia salmonis and associated phage particles

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 44:231-235 (2001) - doi:10.3354/dao044231 Purification of Piscirickettsia salmonis and associated phage particles Sema Akinlar Yuksel1, Kim D. Thompson1,*, Anthony E. Ellis2, Alexandra Adams1 1Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK 2RS Marine Laboratory, Victoria Road, Aberdeen AB11 9DB, Scotland, UK *Corresponding author. E-mail: kdt1@stir.ac.uk ABSTRACT: Piscirickettsia salmonis was isolated from cell culture using differential centrifugation and purified on a 30% Percol gradient. The purity of the preparation was assessed by transmission electron microscopy and phage-like particles were found to be associated with some of the P. salmonis isolates examined. This is believed to be the first report of a phage associated with rickettsia from fish. KEY WORDS: Piscirickettsia salmonis · Rickettsia · Purification · Bacteriophage Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 44, No. 3. Online publication date: April 10, 2001 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2001 Inter-Research.