Hapten Synthesis for a Monoclonal Antibody Based ELISA for Deltamethrin

An enzyme-linked immunosorbent assay (ELISA) was developed for the insecticide deltamethrin. Two haptens were synthesized: the first one was a derivative of the whole molecule with a spacer arm bound to the aromatic ring; the second one was a derivative of deltamethric acid. Twelve monoclonal antibodies were obtained. A competitive ELISA using monoclonal antibody Del 01 yielded the most sensitive assay. The IC 50 value for deltamethrin was estimated to be 0.5 μg mL - 1 with a detection limit of 0.08 μg mL - 1 . Monoclonal antibody Del 01 seems to be deltamethrin specific since <1% cross-reactivity with other pyrethroid analogues was observed.