Key result
In vitro photo-crosslinking revealed five contacting proteins in the U5 snRNP, with Prp8p interacting extensively with the 5' stem-loop to potentially stabilize loop 1-exon interactions.
Population
Saccharomyces cerevisiae U5 snRNP reconstituted in vitro
Design
Preclinical
Authors
Loading...
Prp8p-U5 contacts may anchor exons in spliceosome; leaves open in vivo validation and disease relevance.
The extensive contacts between Prp8p and the 5' stem-loop of U5 RNA suggest Prp8p may be the principal anchoring factor for exons in the spliceosome.
Dix et al. (1998) studied this question. In vitro photo-crosslinking was evaluated on Protein-RNA interactions in the U5 snRNP. In vitro photo-crosslinking revealed five contacting proteins in the U5 snRNP, with Prp8p interacting extensively with the 5' stem-loop to potentially stabilize loop 1-exon interactions.
Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context: