Key result
Phylogenetic analysis of 18 rotavirus A G3P[6] strains from children with acute gastroenteritis revealed a DS-1-like genotype constellation and evidence of equine-like reassortment.
Why the study?
The study was conducted to perform a molecular analysis of rotavirus A G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil.
Observational (n=18)
Equine-like RVA G3P[6] strains are circulating in the Brazilian Amazon, exhibiting amino acid alterations in neutralizing epitopes compared to vaccine strains.
Circulating equine-like G3P[6] strains with epitope changes may signal vaccine mismatch risk; leaves open need for expanded surveillance.
Aim: To perform a molecular analysis of rotavirus A (RVA) G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil. Materials & methods: Eighteen RVA G3P[6] strains were collected from children aged under 10 years hospitalized with acute gastroenteritis, and partial sequencing of each segment genome was performed using Sanger sequencing. Results: Phylogenetic analysis showed that all G3P[6] strains had a DS-1-like genotype constellation. Two strains had the highest nucleotide identities with equine-like G3P[6]/G3P[8] genotypes. Several amino acid alterations in VP4 and VP7 neutralizing epitopes of equine-like RVA G3P[6] strains were observed in comparison with vaccine strains. Conclusion: These findings suggest that equine-like RVA G3P[6] strains have been circulating in the Amazon region of Brazil as a result of direct importation, and support natural RVA evolutionary mechanisms.
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Serra et al. (2021) conducted an observational in Acute gastroenteritis (n=18). Rotavirus A G3P[6] infection was evaluated on Genotype constellation and nucleotide identities. Phylogenetic analysis of 18 rotavirus A G3P[6] strains from children with acute gastroenteritis revealed a DS-1-like genotype constellation and evidence of equine-like reassortment.
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