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Up to the present time pectin in cell-walls has been regarded as amorphous and optically isotropic. However, by means of polarization microscopy, X-ray diffraction, and electron microscopy, it is here demonstrated that the pectin in the fresh collenchyma cell-walls of Petasites vulgaris petioles consists of axially oriented submicroscopic fibrils containing axially oriented pectin crystallites and exhibiting negative intrinsic birefringence.
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Roelofsen et al. (1951) studied this question.
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