Chicken anaemia virus influences the pathogenesis of Marek's disease in experimental infections, depending on the dose of Marek's disease virus

Eight groups of 1-day-old chickens were inoculated with 0, 250, 5000, or 100,000 white blood cells of chickens infected with Marek's disease virus strain K (MDV-WBC). Four of these groups were additionally infected with 10(5) TCID50 chicken anaemia virus (CAV). At day 14 after inoculation, chickens infected with CAV had reduced haematocrit levels, reduced body weights, and depletion of the thymic cortex and bone marrow. Semi-quantitative immunohistochemical examination of nerves and visceral organs was performed at day 28 by immunoperoxidase staining in which a monoclonal antibody specific for leucocytes was used. CAV significantly enhanced the number of lymphoproliferative lesions induced by 5000 MDV-WBC. In contrast, CAV significantly reduced the number of lymphoproliferative lesions induced by 100,000 MDV-WBC. Comparable results were found at day 61 after macroscopic examination of nerves and visceral organs. These findings show that the pathogenesis of MD in experimental infections appears to be enhanced or inhibited by CAV, depending on the dose of MDV.