Tissue Culture Studies With Infectious Hepatitis Specimens

Twenty-nine human adult and eight monkey tissues were studied in culture. The addition of prednisolone to growth media restricted the growth to epithelial-like cells and also provided a slight growth stimulus. Thirty-four specimens of acute hepatitis origin failed to provide indications of virus. Detroit-6 cells were cultured in synthetic media supplemented with sheep serum. Sixteen experiments were inoculated with 82 specimens of acute hepatitis origin. There were no conclusive results with this line of cells.