Key result
Sequence analysis of rotavirus strains from 7 viraemic children revealed discrepancies between faecal and blood VP7 and VP4 amino acid sequences, suggesting preferential extraintestinal dissemination.
Observational (n=7)
Sequence analysis suggests that specific rotavirus variants may preferentially disseminate extraintestinally during co-infection.
May indicate strain-specific extraintestinal rotavirus spread in children; hypothesis-generating and requires validation before clinical consideration.
Information on genotypes or variants associated with systemic rotavirus infection is lacking. Seven viraemic children with acute rotavirus diarrhoea were evaluated. All faecal strains were genotyped (5 G1P[8] type, 1 G9P[8], 1 G1-G4P[8]). Only 3 of 7 blood strains were typeable (G1P[8], G4P8, and G1P[ND]). A discrepancy between faecal and blood VP7 amino acid sequences (Ser-->Asp at position 94) was found in the first child. Two amino acid substitutions (Ile-->Val and Asn-->Ser at position 106 and 113) in the VP4 sequences were demonstrated in the second child. Complete correspondence was found in the third child. All of the observed amino acid substitutions may be involved in rotavirus neutralisation. We speculate that during co-infection by multiple strains, a preferential extraintestinal dissemination of some variants may occur, possibly because of differential viral characteristics.
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Chiappini et al. (2009) conducted an observational in Viraemia and acute rotavirus diarrhoea (n=7). Rotavirus infection was evaluated on VP7 and VP4 amino acid sequences in faecal versus blood strains. Sequence analysis of rotavirus strains from 7 viraemic children revealed discrepancies between faecal and blood VP7 and VP4 amino acid sequences, suggesting preferential extraintestinal dissemination.
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