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The ferrocene-pyrene dyad 3 is able to selectively sense the pyrophosphate anion. The anion recognition was evaluated using electrochemistry, (1)H NMR, as well as fluorescence spectroscopy. The binding event can be inferred from either the redox-shift (DeltaE(1/2) = -100 mV) or the emission intensity ratio of the pyrene monomer to the excimer emission bands in both the neutral and oxidized forms of the receptor upon complexation.
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Romero et al. (2009) studied this question.
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