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Callus tissue was induced on parenchyma tissue of shoot apices, leaves, and inflorescences of Saccharum sp. using a basal mineral medium supplemented with coconut water (10% by volume) and 2,4‐D. Transfer of callus tissue to a non‐2,4‐D basal medium induced the differentiation of plants. In all the plants studied, morphological variants from the parental clone were observed.
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Heinz et al. (1969) studied this question.
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